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What is Cryopreservation? Dr. Hrishikesh Pai



If you are a person who wants to have children but wants to prioritize your education or career first, then cryopreservation is one of the best methods to ensure that you can get pregnant later in life. 

In this article, Dr. Hrishikesh Pai, one of the best IVF doctor in Nagpur will discuss the different  types and methods and some of the advantages of cryopreservation.

Read on to know more about the article,

What is cryopreservation?

Cryo – preservation is the process where cells, tissues, organelles, extracellular matrix, and organs are preserved by cooling to low temperatures.

For cryopreservation to be used with optimal success it is essential that the entire protocol of freezing, storage, and thawing procedures is carried out in the precisely required and prescribed manner.

 The team concerned with freezing must, therefore, be appropriately equipped, experienced and informed to be able to preserve the maximum viability in the frozen samples. The team responsible for the recovery of this viability, by thawing, need not be specialized in the earlier stages of cryopreservation but must be appropriately skilled and knowledgeable

Two methods of cryopreservation 

The two methods of cryopreservation are:

  1. Slow freezing:

Slow freezing is a technique where the cells are cooled very slowly at around -196 C over the course of several hours. This temperature allows for the frozen eggs to be safely preserved- this temperature allows all the biological functions to stop inside the cell. This process is also called a controlled rate or slow programmable freezing.

Dr. Hrishikesh Pai, from IVF Bloom clinic, which is one among the top ten IVF hospitals in India, says that slow freezing was popular back in the mid-90s, but as of now vitrification is used as the process preserves the cells without any damage

We’ll be seeing about vitrification below,

  1. Vitrification

Vitrification is the opposite of slow freezing. Vitrification is a flash freezing method in which the cells are cooled quickly in a couple of minutes. 

According to studies, vitrification is a more successful method of preserving the cells than slow freezing, as in the slow freezing method, it is likely that ice crystals will form inside the cells. Ice crystals cause damage to the cell structures, and this will make the cell unfunctional after the thawing process. 

Vitrification uses higher concentrations of cryoprotectants (chemical compounds that prevent cells or tissues from damage due to freezing) to freeze the cells faster. This will prevent the cells from being damaged.

Types of freezable tissues

The different types of freezable tissues include;

  1. Egg(oocyte) freezing:

Human oocyte cryopreservation is a procedure to preserve a woman’s eggs. This technique has enabled women to postpone pregnancy to a later date – whether for medical or social reasons. 

Procedure for egg freezing

  • Ovarian stimulation: your doctor will give you drugs/medications that will trigger the ovaries to produce more eggs than usual. The medicines are given two weeks before retrieving the eggs.
  • Egg retrieval: The doctor will give you mild sedation and insert a transvaginal ultrasound into the vagina to locate the follicles. The doctor will place a needle to retrieve the eggs from the follicles. The doctor will extract up to 10-15 egg follicles. 
  • Egg freezing: The retrieved eggs are frozen to a sub-zero temperature to store for future use.
  1. Sperm freezing:

The procedure for sperm freezing is :

  • Your sperm will be collected at the lab.
  • The lab doctor will examine the quality and quantity of the sperm. 
  • The doctor will transfer only the high-quality sperm for the cryopreservation process, where your sperm will be stored for future use.
  1. Embryo cryopreservation:

High-quality embryos are frozen at sub-zero temperatures from fertilization to the blastocyst stage.

Procedure for embryo freezing

  • Your doctor will give you medications to stimulate the production of eggs. This will be done 2-3 weeks before removing the eggs.
  • Your doctor will retrieve the eggs from your follicle and incubate the removed eggs with sperms to fertilize them.
  • The fertilized eggs are cryopreserved after three to five days of fertilization.

Advantages of cryopreservation

Dr. Hrishikesh Pai, one of the best IVF doctors in Mohali, says that cryopreservation can benefit a lot of couples.

  • Delay pregnancy: The majority of women who choose to freeze their eggs prefer to put off having children until their 30s or early 40s. After 30, women’s eggs start to deteriorate in quality and quantity, making it harder to become pregnant and maintain a healthy pregnancy. Women who desire to postpone motherhood for personal reasons should save higher-quality eggs in their 20s.
  • Preimplantation Genetic Testing: Women worried about passing certain genetic disorders onto their children can have embryos undergo genetic testing before they are frozen. Embryos that do not have genetic disorders can then be screened out and used for uterine implantation purposes.
  • Beneficial for Women undergoing cancer treatment: Cancer therapies will negatively impact male and female fertility. Radiation and anti-cancer medications target egg and sperm cells’ DNA, which significantly lowers their viability. Cryopreservation gives women who wish to get pregnant but find out they need extensive cancer therapy the option of freezing eggs that can be used after they finish cancer treatments.
  • Unused frozen embryos for multiple pregnancies: Any extra embryos can be transplanted to boost the likelihood of conception if one frozen embryo transfer doesn’t result in a pregnancy. Dr. Hrishikesh Pai, one of the best IVF doctor in Mohali says only cryopreservation offers the benefit of having numerous frozen embryos ready for usage over an extended period of time. 
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How Much Does It Typically Cost To Settle An Accident Involving A Truck Or 18-Wheeler?




The truth is that there is no “typical” payout for a vehicle accident. The ability of victims of truck accidents to obtain full compensation for their losses is contingent on several factors. These factors include the severity of the victim’s injuries, the degree to which the victims’ contributed to the severity of the crash, and the amount of insurance coverage that is liable to the victims with the assistance of an 18-wheeler accident attorney.

Record-Setting Compensation Awarded For 18-Wheeler Crashes.

Accidents involving 18-wheelers and other large commercial trucks are significantly more likely to produce catastrophic injuries, leading to much greater verdicts and settlements than passenger vehicles. Recoveries in the hundreds of thousands, if not millions, of money, are common for families of people killed in 18-wheeler accidents.

Settlements involving trucks and 18-wheelers have the potential to be unprecedented in size in some circumstances.

Texas’ Highest Truck Accident Payout

As just one example, Auto Accident Lawyers successfully negotiated a $23.5 million settlement for a client who suffered catastrophic injuries after a head-on collision with a commercial truck, making it the largest payment in Texas and the largest in the defendant’s corporate history.

 The client can now afford the finest medical care and secure his financial future for his loved ones.

Accident Involving A Truck In The Oil Industry Costs $32 Million

A client of Truck Accident Attorneys recently received a record-breaking $32 million auto accident payout after a truck driver who had been working too many hours crossed the center median and crashed with the client and his family.

Less than one year after filing a truck accident case on behalf of the client and his family, the attorneys handling the 18-wheeler accident settlement reached a record-setting $32 million.

Family Of 18-Wheeler Victim Gets Record Settlement.

An 18-wheeler murdered another wrongful termination truck accident victim’s husband on his way to work in Jackson County, Texas, and we just reached the record-setting (and secret) payment for her.

The trucking company initially denied responsibility and blamed her husband’s cell phone use. Attorneys for the truck accident not only demonstrated that he had not used his smartphone that morning by collaborating with law enforcement agents, but they also discovered proof that the driver of the 18-wheeler had been on the road for more than 14 hours, which violates the standards governing trucking under the federal government.

A few short weeks later, the trucking firm reached a settlement agreement, and the amount of the confidential payout is still the greatest in the company’s history.

Highest Compensation Awarded To Oakley Freight

A Truck Crash That Can’t Be Beaten On Christmas Eve, a client was rear-ended by a true preoccupied ck driver. The client’s attorneys successfully obtained the largest settlement in the history of Oakley Trucking Company, which was number one.

Despite Oakley’s initial refusal to give more than 125,000 dollars in compensation, our legal team successfully established that the truck driver had been talking on his cell phone just at the time of the accident. More than 25 times Oakley’s initial offer, the matter was settled only six months later.

Musings In Conclusion

Every personal injury lawyer will tell you they help people who have been hurt in truck or 18-wheeler accidents. Still, only a select few have successfully taken on the largest trucking firms in the country and won their clients the largest auto accident verdicts & settlements in history.

Firm’s Undefeated Houston 18-Wheeler Injury Lawyers have successfully handled thousands of people injured in truck and commercial vehicle accidents in Houston, Texas, New Mexico, and the United States. We have won billions of dollars and secured industry-leading settlements and verdicts on their behalf.

Whether you or an adored one were hurt or killed in a trucking accident caused by the negligence of the trucking company or the truck driver, you can rest assured that your case will be handled by a team of highly skilled attorneys who will pursue maximum compensation for your suffering and loss from the trucking company.

If they take your case, you won’t owe them a thing for the consultation or any work they do unless you do.

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3 Online Bingo Reviewed




Playing bingo is an incredible method for investing some energy into clean tomfoolery. Not just that, with bingo, you can bring in cash too that no other diversion could rise to. There is a great deal of UK Online Bingo out there that takes special care of each and every extravagant you have. In this article, we will survey three of the famous UK Online Bingo that took part today. They are Virgin Bingo, Gone Bingo and Wink Bingo.

The first, Virgin Bingo brags of 10 bingo rooms where you can play an assortment of bingo games that are for everybody like 90 balls, 80 balls or even the 75 balls. Likewise, a guest can likewise play the most loved Arrangement or No Arrangement game which has caught numerous clients’ eyes. New players are blessed to receive a large group of elements that isn’t generally found in other UK online bingo, like visit offices and free everyday scratch cards. Each prize isn’t to be laughed at where the big stake can go as high as £10000 for coveralls. Indeed, even week after week games are loads enjoyable to take an interest in. A such week after week games like bingo competitions, penny bingo is a fan number one. For the people who are excessively reluctant to place an opening in their pocket, there is a game called no-lose bingo where the player gets his cash back on the off chance that you lose! Isn’t that an impact? With cards that are just 10p each, playing at Virgin Bingo has become famous far and wide.

The following UK online bingo is Wink Bingo. At Wink Bingo you are in for the most famous and greatest web-based bingo webpage. It isn’t to be expected to know why it has accumulated the consideration of many. Playing at Wink Bingo is fun, easy to use and in particular brings a great deal to the table for new information exchanges as well concerning faithful clients. Another player will be empowered with a welcome reward to play the Twist The Wheel game for a little store of £10 or over. This game will allow you an opportunity to bring back home £15 and £1000 by simply being a first-time client! At Wink bingo, the UI is exceptionally simple to utilize and there are no convoluted buttons or guidelines. It utilizes the Globalcom stage to guarantee that you get games that are first-rate in quality and worth.

Every CM is agreeable and is delighted to assist you with your questions whenever. You won’t ever feel like an outsider at Wink bingo. This UK online bingo offers the best and most pursued include called group bingo. Here you are given boatloads of bingo extra focuses with the goal that the more you play, the more focus you get for your group and the more companions you collect. In the event that that doesn’t tempt you, there is likewise something for faithful clients. Each reliable part gets a 10% cashback for many weeks for the misfortunes you might have caused from the stores you have made for that week. Besides, every time you purchase cards or play bingo or even welcome your companions at Wink bingo you gain focuses in a split second as a feature of their devotion conspires.

As is commonly said, the latter isn’t the most un-all of the time. Gone Bingo certain possesses all the necessary qualities. It is a UK online bingo which has won the New Web site of Year for 2007. In 2008 alone it had the option to offer nearly £700K for its supporters! That is obviously an extraordinary method for treating your clients in our book. The tremendous assortment of games at Gone bingo implies that they have everything for each player out there. There is 90 ball, 80 balls,75 balls and 30 ball bingo. In addition, the 3-reel and 5-reel spaces have taken the extravagant of many. Most bingo rooms are full on some random day and that implies you can make many companions en route. The Talk aces are proficient and prepared to take care of you whenever. In particular, this UK online bingo has client care day in and day out throughout the entire year so you realize you are in safe hands. There you go three of the most well-known UK online bingo locales out there. Everyone has the word FUN illuminated for its clients.

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Grab The Most Advanced Benefits Of Crypto Tax Wallet Tracker




Do you want to know the crypto tax wallet tracker? Then proceed with this article now. The crypto tax wallet tracker generally consists of the most advanced tracker, which can be installed inside the crypto tax wallet. You can track it by using the best tracking software.

Use best crypto tax wallet tracking technology:

On the other hand, the world population has adopted crypto tax wallets to access precise tracking technology. It is suitable for commercial and revenue share continues to lead the way for evaluating distance at reviews of the crypto tax wallet tracker. 

Moreover, this consists of the user average and involves the traffic overtaking the crypto tax wallet for the first time. With this crypto tax wallet, the growth of the crypto tax wallet tracker has focused on the users. 

It consists of average users and is helpful for the first time by increasing the first time in the mobile comparison used for the crypto tax wallet of clear. It is essential to say their behaviour from country to country with the right individuals. 

Of course, this is especially seen in customers who remain the critical solution for crypto tax wallet results. It remains customers of individuals to reach their behaviour to the people with the most significant space for customers. 

Best crypto tax wallet tracker:

With the tracker, it creates the best solution to the crypto tax wallet reach without any hassles. It consists of the largest customers by the essential reviews of the crypto tax wallet tracker on choosing an experienced solution in the space. 

It describes responsiveness for everything software to ensure responsive design for crypto tax wallet users. It ensures the performance of various crypto tax wallet users and purchase decisions suitable for people involved in the crypto tax wallet site. It involves high-value purchases booked on the crypto tax wallet but still mobile search forever. 

Obtaining clear performance:

It is suitable for obtaining precise performance and various crypto tax wallet users, depending on the category. It is still looking for high-value purchases for the first start-up involved in the crypto tax wallet tracker values. 

Moreover, this is still a riding share sector that met critical mass, and many choose to be humble to reviews of the crypto tax wallet tracker. It still looks and fails to fast and learn quickly to view the company’s technology development.  


Impact of crypto tax wallet:

It concludes that the rideshare sector that has yet to develop with the order still considers itself to be starting up. It consists of average results and hence gets the high importance of foreseeable future results. The crypto tax wallet tracker is a provider, and you can check out their reviews here. It is vital for the first time by the end of this year that the first time the traffic overtakes the crypto tax wallet continues.  

Here you can find the most advanced process of using the crypto tax wallet tracker. Everyone uses this tracker now without any hesitation. 

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