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What is Cryopreservation? Dr. Hrishikesh Pai



If you are a person who wants to have children but wants to prioritize your education or career first, then cryopreservation is one of the best methods to ensure that you can get pregnant later in life. 

In this article, Dr. Hrishikesh Pai, one of the best IVF doctor in Nagpur will discuss the different  types and methods and some of the advantages of cryopreservation.

Read on to know more about the article,

What is cryopreservation?

Cryo – preservation is the process where cells, tissues, organelles, extracellular matrix, and organs are preserved by cooling to low temperatures.

For cryopreservation to be used with optimal success it is essential that the entire protocol of freezing, storage, and thawing procedures is carried out in the precisely required and prescribed manner.

 The team concerned with freezing must, therefore, be appropriately equipped, experienced and informed to be able to preserve the maximum viability in the frozen samples. The team responsible for the recovery of this viability, by thawing, need not be specialized in the earlier stages of cryopreservation but must be appropriately skilled and knowledgeable

Two methods of cryopreservation 

The two methods of cryopreservation are:

  1. Slow freezing:

Slow freezing is a technique where the cells are cooled very slowly at around -196 C over the course of several hours. This temperature allows for the frozen eggs to be safely preserved- this temperature allows all the biological functions to stop inside the cell. This process is also called a controlled rate or slow programmable freezing.

Dr. Hrishikesh Pai, from IVF Bloom clinic, which is one among the top ten IVF hospitals in India, says that slow freezing was popular back in the mid-90s, but as of now vitrification is used as the process preserves the cells without any damage

We’ll be seeing about vitrification below,

  1. Vitrification

Vitrification is the opposite of slow freezing. Vitrification is a flash freezing method in which the cells are cooled quickly in a couple of minutes. 

According to studies, vitrification is a more successful method of preserving the cells than slow freezing, as in the slow freezing method, it is likely that ice crystals will form inside the cells. Ice crystals cause damage to the cell structures, and this will make the cell unfunctional after the thawing process. 

Vitrification uses higher concentrations of cryoprotectants (chemical compounds that prevent cells or tissues from damage due to freezing) to freeze the cells faster. This will prevent the cells from being damaged.

Types of freezable tissues

The different types of freezable tissues include;

  1. Egg(oocyte) freezing:

Human oocyte cryopreservation is a procedure to preserve a woman’s eggs. This technique has enabled women to postpone pregnancy to a later date – whether for medical or social reasons. 

Procedure for egg freezing

  • Ovarian stimulation: your doctor will give you drugs/medications that will trigger the ovaries to produce more eggs than usual. The medicines are given two weeks before retrieving the eggs.
  • Egg retrieval: The doctor will give you mild sedation and insert a transvaginal ultrasound into the vagina to locate the follicles. The doctor will place a needle to retrieve the eggs from the follicles. The doctor will extract up to 10-15 egg follicles. 
  • Egg freezing: The retrieved eggs are frozen to a sub-zero temperature to store for future use.
  1. Sperm freezing:

The procedure for sperm freezing is :

  • Your sperm will be collected at the lab.
  • The lab doctor will examine the quality and quantity of the sperm. 
  • The doctor will transfer only the high-quality sperm for the cryopreservation process, where your sperm will be stored for future use.
  1. Embryo cryopreservation:

High-quality embryos are frozen at sub-zero temperatures from fertilization to the blastocyst stage.

Procedure for embryo freezing

  • Your doctor will give you medications to stimulate the production of eggs. This will be done 2-3 weeks before removing the eggs.
  • Your doctor will retrieve the eggs from your follicle and incubate the removed eggs with sperms to fertilize them.
  • The fertilized eggs are cryopreserved after three to five days of fertilization.

Advantages of cryopreservation

Dr. Hrishikesh Pai, one of the best IVF doctors in Mohali, says that cryopreservation can benefit a lot of couples.

  • Delay pregnancy: The majority of women who choose to freeze their eggs prefer to put off having children until their 30s or early 40s. After 30, women’s eggs start to deteriorate in quality and quantity, making it harder to become pregnant and maintain a healthy pregnancy. Women who desire to postpone motherhood for personal reasons should save higher-quality eggs in their 20s.
  • Preimplantation Genetic Testing: Women worried about passing certain genetic disorders onto their children can have embryos undergo genetic testing before they are frozen. Embryos that do not have genetic disorders can then be screened out and used for uterine implantation purposes.
  • Beneficial for Women undergoing cancer treatment: Cancer therapies will negatively impact male and female fertility. Radiation and anti-cancer medications target egg and sperm cells’ DNA, which significantly lowers their viability. Cryopreservation gives women who wish to get pregnant but find out they need extensive cancer therapy the option of freezing eggs that can be used after they finish cancer treatments.
  • Unused frozen embryos for multiple pregnancies: Any extra embryos can be transplanted to boost the likelihood of conception if one frozen embryo transfer doesn’t result in a pregnancy. Dr. Hrishikesh Pai, one of the best IVF doctor in Mohali says only cryopreservation offers the benefit of having numerous frozen embryos ready for usage over an extended period of time. 
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Workers Comp in California FAQ: Answering Common Questions




Are you a business owner considering worker’s compensation insurance but have questions about what it is, how to get it, and how much it may cost? If so, then you’re in the right place. 

In this blog post, we’ll answer your common questions about worker’s comp and help you better understand all that goes into obtaining coverage for your employees.

By reading this workers’ comp in California FAQ, we hope you will feel more comfortable and confident when making decisions about protecting your staff with insurance. So let’s dive in.

Workers’ compensation is a system of insurance that benefits employees injured or who become ill due to their job.. Workers’ compensation is mandatory in most states, meaning employers must provide coverage for their employees.

  • What Does Workers’ Comp Cover?

This workers comp in California FAQ helps inform you of important protection for employers and employees. This insurance covers medical expenses related to injuries, lost wages, and rehabilitation costs. Additionally, worker’s compensation can help cover disability benefits for individuals who cannot return to work due to injury or illness.

Moreover, death benefits are paid if an employee passes away due to their work on the job. Overall, worker’s compensation offers protection during tough times and provides a financial cushion for those affected by workplace injuries or illnesses.

  • How Much Does Workers’ Comp Cost?

Workers’ comp premiums are complex and can vary from one business to the next. An employer’s industry, payroll size, rate schedule, and experience rating primarily influence these premiums’ costs. In addition, industry-specific factors like insurance losses compared to industry standards can also affect worker’s comp premiums.

Companies with well-managed safety practices will often have lower premium rates, as worker’s comp claims are more difficult for insurers to deny when businesses practice preventative safety methods. Payroll size also plays an influential role. For example, companies with fewer employees tend to be viewed by insurers as having higher risks, thereby increasing their premiums.

  • Who Is Required To Hold Workers’ Comp Insurance?

California businesses with at least one employee other than the owner must obtain workers’ compensation insurance. Employers can avoid fines and penalties for breaking California labor law with this coverage. Additionally, failing to provide workers’ comp can prevent an employer from securing future business licenses.

Avoiding worker’s comp insurance requirements may also expose businesses to costly civil lawsuits from employees who suffer from workplace-related injuries but cannot secure benefits. All California businesses with employees must secure proper worker’s compensation insurance for these reasons.

Conclusion: California Business Owners Need Workers’ Comp Coverage

Workers’ compensation insurance is vital for any business that employs people. If you have employees, you are required to carry workers comp insurance. The cost of worker’s comp varies depending on your state and industry, but it’s an important expense to factor into your budget.

If you need assistance with obtaining worker’s comp, there are a variety of reputable brokers who can get your business the coverage that’s right for your business. We hope this workers comp in California FAQ has given you more confidence when deciding which policy is right for your business.


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How Many Boats Do You Need?




You might think you need a boat to fish in your local river, but that’s not the case. You don’t even need a fishing rod and line—you can just use a fishing net! This is an amazing revelation, and it comes about because of something called drag. Drag means that when you put your weight into the water, it pulls the prey towards the boat. And since boats have more weight than other objects in the water, they pull more prey towards them than others objects. This is why sometimes you can fish from a boat—you can easily get close to your prey without having to worry about their size or speed. BoatOutfitters

How to Choose the Right Boat for Your Season.

Boats play an important role in many aspects of life, including travel. For example, a boat can be used for fishing, kayaking, sailing, and swimming. In addition, boats can be used to transport people and cargo.

Different types of boats are available for different purposes. For instance, a small yacht might be used for short trips in the water or as a leisure vessel while a large cruise liner could serve as the primary transportation for groups or an entire country.

A boat’s purpose is also determined by its size. A smaller boat such as a canoe is perfect for shorter trips in rivers or lakes while a larger boat such as a yacht can carry more people and cargo.

There are also different types of watercraft available that are specific to certain activities or regions. For example, catamarans are designed for sailing while sailboats are designed to use sails to move through the water.

How to Get the Most Out of Your Boat rental.

To get the most out of your boat rental, rent as soon as possible. This will give you the best deal and allow you to take your time exploring the area. You can also book a longer-term rental for a more relaxed experience.

Book a Boat rental for a longer period of time.

If you want to explore an area for a longer period of time, Book your rental in advance rather than let it fill up quickly. This will ensure that you have plenty of space to enjoy your vacation without feeling crowded or rushed.

Use the Right Boat for the Right Season.

It’s important to choose the right boat for the right season – if you’re planning on sailing in warm weather, consider renting a fiberglass boat; if you’re looking to go ice-boating, choose a wooden boat; and so on. By knowing which type of boat is best suited for your needs, you can save money and have a great experience on your vacation rentals!

Tips for Renting a Boat.

Before renting a boat, be sure to know the regulations of your country. Many rental companies will require you to have a contract in place with them before beginning the rental process. Additionally, make sure you understand the boat’s specifications and what climate control it features.

Get a Boat with a Climate Control.

If you want to rent a boat that has climate control, be sure to get one before rent begins. This will ensure that your boat is kept cool during hot summer days or cold winter nights. And while refrigerators are not traditionally allowed on boats (although some companies may provide adapters for this), it’s always ideal to have food and other supplies ready in case of an emergency.

Have a Contract with the Boat Rental Company.

Make sure you have a contract in place with the rental company so that you can accurately anticipate your obligations and receive payment for your rented boat on time. Contracts also help protect both you and the company from any potential issues down the road.

Renting a boat can be an amazing experience, but it’s important to take some precautions while renting. By knowing the regulations of your country and having a contract with the rental company, you can ensure that your experience is safe and hassle-free. Finally, Tips for Renting a Boat should help make sure that you have an amazing time boating around your local area.

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Choosing the most suitable limousine




When looking for the limousine that best suits your needs, you will need to take into consideration a number of other factors. Some industry experts are of the opinion that customers are not always sufficiently informed before making use of this kind of service.


According to these experts, customers should take into account the event for which they require it, the number of people, the hours, or the distance to the one that is located before making their reservation.


Putting the finishing touches on the reservation for the limousine.

Since many businesses charge on an hourly basis, it is vital to plan events and perform the appropriate calculations in order to determine how much time is required for the space that we wish to hire.


Value for money

There are many different kinds of Executive limo service, so cost should not be the only thing you consider when making your choice. There are numerous kinds of services, much like there are hotels.


Protection is essential.

It is in everyone’s best interest for the taxi business that is providing the service to have a variety of assurances, including an appropriate registration, license, and insurance policy. This will ensure that both you and the group are in capable hands.


There is a limousine reserved for each level of service.

It may be more convenient to use one service over another, depending on the nature of the service that is being provided.


Book in due time

This is especially important for occasions in which we will want more than one vehicle, since it will be required to make reservations in plenty of time in advance for those occasions.


When it comes to weddings, it is preferable to do a calculation with a prediction that can range anywhere from 12 to 18 months, but in average, they are requested anywhere from three to four weeks in advance.


When making a reservation, it is in your best interest to obtain confirmation in writing. Additionally, it is recommended that you contact within a week of the reservation to ensure that everything is in order and to confirm the reservation.


Limousines and conventions come to mind.

There are several cities that frequently host huge conventions or events, which may imply that the demand in question may be exhausted on particular days.


As a result, if we are aware of what we are going to require, we will have to pay close attention to the details and accordingly plan ahead when we are hiring them.

It is necessary for you to consider each of the companies’ proposals.

It is easy to locate numerous businesses on the internet; hence, getting in touch with them is always a smart idea, and they will provide you with all of the information that you require.


In this context, it is important not to forget that the level of attention that may be obtained over the phone undoubtedly reveals a great deal about the kind of business being dealt with.


Always ask whatever you are going to want without fear, and when replying to requests, always check the treatment that is being offered.


It is usually interesting to enquire about the fees, as well as whether there is a deposit or cancellation fee for the services, as well as whether there is a fee for certain extra services.


This vehicle’s operator

It is necessary to determine or make the decision based on the parameters that can be obtained or compared, selecting the parameter that, in our opinion, generates the greatest amount of confidence. Something really significant with reference to the driver.


It will not be simple to receive information about the driver because there are many instances in which it is possible that they will assign us to one or the other depending on the logistical need that exists at the present time; however, whenever it is possible, it is preferable to inform yourself.


Sometimes there are certain prerequisites that need to be satisfied, such as being able to speak a certain language or being familiar with the ins and outs of the place.


Although it is significant, price is not the only item to consider.

Although the cost becomes of significant relevance, providing adequate service is of even greater significance. Do not make the decision to go with the option that has the cheapest rate right away since you can find yourself riding in a limousine from the 1980s with a driver who has a poor reputation.


Make a reservation for the limousine.

At the time that we make the reservation, it is in our best interest to make certain that we have a copy of the contract and the reservation number, as well as that the cancellation policy is reflected, both in writing and by email, in addition to the payment and the signal. This is the best way to ensure that we meet all of the requirements for making the reservation.


You can call a few days ahead of time so that it can be confirmed, and you can also request the personal mobile number of whoever is going to be the driver of the limousine. This will allow you to check the specifics and find out if you have all of the necessary information, just in case there is something that has not been provided.


Businesses that act as go-betweens

In the market for Chicago limo service, there are some organizations whose business model consists of transferring employment from one company to another based on the likelihood of being able to satisfy the requirements of the market at the time. Checking to see if something like this is possible with your own reservation is an important thing to do. In the event that it arrives late, do not be afraid to double check the particulars, to ensure that there are no misunderstandings, and to determine whether or not it is conceivable that the new company will fulfill all of your requirements.

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