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What is Cryopreservation? Dr. Hrishikesh Pai

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If you are a person who wants to have children but wants to prioritize your education or career first, then cryopreservation is one of the best methods to ensure that you can get pregnant later in life. 

In this article, Dr. Hrishikesh Pai, one of the best IVF doctor in Nagpur will discuss the different  types and methods and some of the advantages of cryopreservation.

Read on to know more about the article,

What is cryopreservation?

Cryo – preservation is the process where cells, tissues, organelles, extracellular matrix, and organs are preserved by cooling to low temperatures.

For cryopreservation to be used with optimal success it is essential that the entire protocol of freezing, storage, and thawing procedures is carried out in the precisely required and prescribed manner.

 The team concerned with freezing must, therefore, be appropriately equipped, experienced and informed to be able to preserve the maximum viability in the frozen samples. The team responsible for the recovery of this viability, by thawing, need not be specialized in the earlier stages of cryopreservation but must be appropriately skilled and knowledgeable

Two methods of cryopreservation 

The two methods of cryopreservation are:

  1. Slow freezing:

Slow freezing is a technique where the cells are cooled very slowly at around -196 C over the course of several hours. This temperature allows for the frozen eggs to be safely preserved- this temperature allows all the biological functions to stop inside the cell. This process is also called a controlled rate or slow programmable freezing.

Dr. Hrishikesh Pai, from IVF Bloom clinic, which is one among the top ten IVF hospitals in India, says that slow freezing was popular back in the mid-90s, but as of now vitrification is used as the process preserves the cells without any damage

We’ll be seeing about vitrification below,

  1. Vitrification

Vitrification is the opposite of slow freezing. Vitrification is a flash freezing method in which the cells are cooled quickly in a couple of minutes. 

According to studies, vitrification is a more successful method of preserving the cells than slow freezing, as in the slow freezing method, it is likely that ice crystals will form inside the cells. Ice crystals cause damage to the cell structures, and this will make the cell unfunctional after the thawing process. 

Vitrification uses higher concentrations of cryoprotectants (chemical compounds that prevent cells or tissues from damage due to freezing) to freeze the cells faster. This will prevent the cells from being damaged.

Types of freezable tissues

The different types of freezable tissues include;

  1. Egg(oocyte) freezing:

Human oocyte cryopreservation is a procedure to preserve a woman’s eggs. This technique has enabled women to postpone pregnancy to a later date – whether for medical or social reasons. 

Procedure for egg freezing

  • Ovarian stimulation: your doctor will give you drugs/medications that will trigger the ovaries to produce more eggs than usual. The medicines are given two weeks before retrieving the eggs.
  • Egg retrieval: The doctor will give you mild sedation and insert a transvaginal ultrasound into the vagina to locate the follicles. The doctor will place a needle to retrieve the eggs from the follicles. The doctor will extract up to 10-15 egg follicles. 
  • Egg freezing: The retrieved eggs are frozen to a sub-zero temperature to store for future use.
  1. Sperm freezing:

The procedure for sperm freezing is :

  • Your sperm will be collected at the lab.
  • The lab doctor will examine the quality and quantity of the sperm. 
  • The doctor will transfer only the high-quality sperm for the cryopreservation process, where your sperm will be stored for future use.
  1. Embryo cryopreservation:

High-quality embryos are frozen at sub-zero temperatures from fertilization to the blastocyst stage.

Procedure for embryo freezing

  • Your doctor will give you medications to stimulate the production of eggs. This will be done 2-3 weeks before removing the eggs.
  • Your doctor will retrieve the eggs from your follicle and incubate the removed eggs with sperms to fertilize them.
  • The fertilized eggs are cryopreserved after three to five days of fertilization.

Advantages of cryopreservation

Dr. Hrishikesh Pai, one of the best IVF doctors in Mohali, says that cryopreservation can benefit a lot of couples.

  • Delay pregnancy: The majority of women who choose to freeze their eggs prefer to put off having children until their 30s or early 40s. After 30, women’s eggs start to deteriorate in quality and quantity, making it harder to become pregnant and maintain a healthy pregnancy. Women who desire to postpone motherhood for personal reasons should save higher-quality eggs in their 20s.
  • Preimplantation Genetic Testing: Women worried about passing certain genetic disorders onto their children can have embryos undergo genetic testing before they are frozen. Embryos that do not have genetic disorders can then be screened out and used for uterine implantation purposes.
  • Beneficial for Women undergoing cancer treatment: Cancer therapies will negatively impact male and female fertility. Radiation and anti-cancer medications target egg and sperm cells’ DNA, which significantly lowers their viability. Cryopreservation gives women who wish to get pregnant but find out they need extensive cancer therapy the option of freezing eggs that can be used after they finish cancer treatments.
  • Unused frozen embryos for multiple pregnancies: Any extra embryos can be transplanted to boost the likelihood of conception if one frozen embryo transfer doesn’t result in a pregnancy. Dr. Hrishikesh Pai, one of the best IVF doctor in Mohali says only cryopreservation offers the benefit of having numerous frozen embryos ready for usage over an extended period of time. 
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How to Scale Your Brand with Little Budget: Advice from a Marketing Expert

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Scaling a brand doesn’t have to drain your wallet. In fact, some of the most effective strategies are low-cost and high-impact—as long as they’re applied with precision and intention.

Adrián Rubio, founder of Arubeus, a digital marketing agency that helps startups and growing businesses thrive, shares proven tactics that any entrepreneur can implement—no matter how limited the budget.

1. Define a laser-focused niche

Trying to appeal to everyone is one of the fastest ways to burn money. Instead, hone in on a very specific audience segment.

A well-defined niche allows you to craft targeted content, speak directly to your ideal client’s pain points, and build trust faster. Whether it’s “ethical skincare for men” or “freelance tools for architects,” a niche gives you a strategic advantage over generic competitors.

“Broad messaging is expensive and inefficient,” says Rubio. “Niche targeting increases conversions and loyalty.”

2. Leverage organic content to build visibility

Content is still king—but only when it’s consistent and valuable. You don’t need a huge ad budget to be visible. Focus on:

  1. Blog posts optimized for SEO
  2. Short, authentic videos on platforms like TikTok or Instagram Reels
  3. Educational carousels or infographics on LinkedIn or Instagram

This kind of organic content builds brand awareness and positions you as an authority—without spending a dime.

3. Use smart tools (many are free or affordable)

You don’t need an enterprise tech stack to compete. Powerful free and low-cost tools can handle everything from automation to analytics. A few favorites:

  1. Mailchimp for email marketing
  2. Canva for design
  3. Google Search Console for SEO insights
  4. Metricool or Buffer for social media scheduling

“The key isn’t how much you spend,” says Rubio, “but how well you execute with what you have.”

4. Collaborate with micro-influencers

Celebrity endorsements are out of reach for most startups, but micro-influencers (with 5,000–50,000 followers) offer a sweet spot. They often have more engaged, loyal audiences—and are open to collaborations, shout-outs, or product exchanges.

These partnerships can expand your reach organically and build credibility in a targeted community.

5. Build an email list from day one

Social media followers come and go, but an email list is an owned asset. Start building your list early by offering a lead magnet—like a checklist, discount code, or free resource.

Then, nurture your subscribers with helpful, high-value content. Email marketing consistently delivers one of the highest ROI across all digital strategies.

Conclusion

Scaling your brand on a small budget is 100% possible. It takes clarity, creativity, and a commitment to consistency. By focusing on niche positioning, organic content, smart tools, and relationship-driven tactics, even the leanest startup can grow into a powerful brand.

Want help crafting a strategy that works with your budget? Visit Arubeus.com and discover how Adrián Rubio and his team empower small businesses to grow smarter, not just bigger.

 

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Navigating Insurance Challenges After a Car Accident in Alpharetta, GA

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Dealing with insurance after a car accident in Alpharetta, GA can feel overwhelming. Insurance companies often complicate claims. You might face delays, disputes, and denials. This process is stressful when you already have injuries or car damage. You need clear guidance to handle these challenges effectively. Knowing your rights and understanding policy terms can bring much-needed clarity. That’s where a car accident attorney can help. They can make sure you receive the compensation you deserve and simplify the complex steps involved. They know the local laws and can handle the tough conversations with insurers. This support lets you focus on recovery rather than worry about paperwork and negotiations. Remember, you don’t have to navigate this alone. Seeking expert help can make this journey less daunting. Your peace of mind matters. We’re here to empower you with the right tools and advice at every step.

Understanding Your Insurance Policy

Before an accident occurs, take time to understand your insurance policy. Knowing the coverage options, deductibles, and limits can help you make informed decisions after an accident. Familiarize yourself with common terms like “liability,” “collision,” and “comprehensive.” This knowledge reduces confusion and helps you navigate claims more easily. Reviewing your policy with an expert or through resources like USA.gov Insurance can provide further insight.

Filing a Claim: The Basics

When you need to file a claim, start by gathering necessary information. This includes the accident date, location, and details of all parties involved. Take photos of the scene and get witness contacts if possible. Notify your insurer promptly, as delays can affect your claim. Always keep a detailed record of your communications with the insurance company. This documentation can be crucial if disputes arise.

Common Insurance Challenges

Many face challenges like claim denials, delayed payments, or insufficient offers. Understanding why these issues occur can help. Common reasons include filing errors, lack of documentation, or disputes over fault. If an insurance company denies your claim, request a detailed explanation in writing. This can form the basis for further discussions or appeals.

Role of a Car Accident Attorney

Attorneys play a vital role in navigating insurance challenges. They understand local laws and can manage communications with insurers effectively. An experienced attorney assesses your case, provides legal advice, and represents your interests. This ensures you receive fair compensation for your losses. For more legal resources and support, refer to the Georgia Government Legal Assistance.

Comparison Table: Handling Challenges Alone vs. With an Attorney

Aspect Handling Alone With an Attorney
Understanding Policy Confusing, time-consuming Clear guidance
Filing Claim Risk of errors Accurate and timely
Dealing with Insurers Overwhelming Professionally managed
Receiving Compensation Delayed or reduced Fair and prompt

Steps to Ensure a Smooth Claim Process

To smoothen your claims process, consider these steps:

  • Review your insurance policy regularly.
  • Keep thorough records of all interactions and documents.
  • Consult with experts early to prevent mistakes.
  • Remain persistent and follow up regularly.

Conclusion

Insurance challenges after a car accident can seem daunting, but you can manage them with the right approach. Understanding your policy, documenting everything, and seeking professional help when needed ensures the best outcomes. You deserve peace of mind and fair compensation. By taking informed steps and possibly consulting a car accident attorney, you protect your rights and recovery. Remember, you’re not alone in this journey. There are resources and professionals ready to support you every step of the way.

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Green Building Starts with Smart Procurement: How Contractors Can Cut Costs and Carbon

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Sustainability in construction starts with smarter procurement. By adopting sustainability procurement for contractors and builders, builders can minimize environmental impact, maximize costs, enhance project efficiency, and reduce costs. Contractors can adhere to green standards while staying within their budget by adopting a variety of strategies, including sourcing materials responsibly and using digital procurement platforms.

This article explores sustainable procurement practices that can help contractors save money while reducing carbon dioxide emissions.

The Role of Smart Procurement in Green Building

Sustainable procurement for contractors prioritizes cost and availability. This approach involves

  • Sourcing with responsibility: Choose suppliers who respect ethical and environmentally friendly standards.
  • Leveraging digital tools: Using purchasing platforms to assess the sustainability credentials and capabilities of suppliers.
  • Optimizing materials use: Reducing landfill waste by choosing energy-efficient, recyclable materials.

By integrating all these factors into the procurement process, contractors can significantly contribute to a carbon-neutral construction industry and improve cost-effectiveness.

Practical Steps to Implement Sustainable Procurement

  1. Prioritize Certified Sustainable Materials

A construction project’s carbon footprint can be reduced by selecting certified sustainable building materials. Check for certifications, including:

  • FSC (Forest Stewardship Council), responsibly sourced wood
  • LEED (Leadership in Energy and Environmental Design) compliant materials
  • Cradle to Cradle Certified TM is a certification for recyclable and eco-friendly products.

By using these materials, you can ensure that your project complies with the green building standard and has a higher sustainability rating.

  1. Digital Procurement Platforms

Digital procurement tools let contractors make data-driven choices about their materials. These platforms assist:

  • Compare sustainability ratings for suppliers
  • Track carbon emissions from material sourcing
  • Identify cost-effective green alternatives

By leveraging technology, contractors can reduce risks associated with procurement, increase efficiency, and achieve sustainability goals.

  1. Smarter Planning Reduces Waste

Sustainable procurement is not just about what materials you buy. Implementing modular construction and just-in-time procurement techniques will help you:

  • Reduce the amount of waste material and excessive use
  • Reduce Transportation Emissions
  • Improve on-site efficiency

Selecting pre-cut, prefabricated, or recycled materials reduces carbon emissions.

  1. Eco-Conscious Suppliers – Join the Eco-Conscious Partner Program

The supply chain will be more environmentally friendly if you select suppliers who share your commitment to sustainability. Contractors should:

  • Assess supplier practices: Check carbon footprints, waste management, and energy efficiency.
  • Negotiate sustainability terms: Encourage bulk purchasing and returnable packaging.
  • Encourage innovations: Work with the manufacturers of low-carbon cement, recycled steel, or bio-based materials.

The development of partnerships with green providers can increase cost savings as well as long-term sustainable efforts.

  1. Consider Energy-Efficient Materials and Recyclables

Building with energy-efficient materials reduces operating emissions and enhances project performance. Consider:

  • Improve energy efficiency with high-performance insulation
  • Cool roofing materials can reduce heat transfer
  • Reduce carbon footprint with recycled or upcycled parts

Circular economy extends the lifecycle of products and materials by reusing or repurposing them. This reduces landfill waste.

Cost Savings Through Sustainable Procurement

In contrast to the popular belief that green building is expensive, contractors who adopt sustainable procurement practices can save significant money by:

  • Smart procurement reduces delays and rework. Many governments provide tax breaks and grant money for green projects.
  • By integrating this strategy, contractors will be able to increase the ROI of their projects while still meeting environmental objectives.

The Future of Green Procurement in Construction

Contractors need to prioritize green procurement as sustainability regulations become stricter and client demands increase. The future lies in green building.

  • AI procurement tools: Automating sustainability assessments
  • Blockchain for Supply Chain Transparency: Ensuring Ethical Sourcing.
  • Carbon neutral Construction Practices: Reducing embodied cadmium through low-impact products.

Contractors who adopt smart procurement strategies today can stay ahead in the industry and build a sustainable, cost-effective tomorrow.

Conclusion

Sustainable construction begins even before the first block is laid. By adopting sustainable contracting, builders can cut costs, reduce emissions, align themselves with green building standards, and adhere to the latest technology. Leveraging digital technologies and partnering with eco-conscious suppliers, a shift towards smart, sustainable purchases is the key to an industry that is greener, more profitable, and more environmentally friendly.

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